PacBio (Pacific Biosciences)

Type: Company (sequencing technology) Ticker: PACB (Nasdaq)

The other half of the long-read duopoly. PacBio's HiFi reads use circular consensus sequencing (CCS) — the same molecule is read in a loop many times and the consensus collapses errors — to deliver the highest long-read accuracy available. Its Revio and Vega systems plus the Kinnex / MAS-ISO-seq library prep make full-length, single-cell isoform sequencing practical at scale.

Key Contributions

• Highest long-read accuracy: 99.9–99.95% — Sequel II 99.9%, Revio 99.95% via circular consensus sequencing. (SCLR-seq review)
• Single-cell isoform throughput — Revio 80–100M HiFi reads/run, Vega 50–60M; MAS-ISO-seq reaches ~40M HiFi reads on Sequel IIe, enabling full-length isoform analysis per single cell via the Kinnex / MAS-seq concatenation approach. (SCLR-seq review)

Mentioned In

• Long-Read Sequencing — PacBio HiFi is the accuracy benchmark and the single-cell isoform workhorse.

Related Entities

• Oxford Nanopore — the other half of the long-read duopoly (nanopore, portable, direct-RNA).